Stanford scientists use optogenetics to effectively stimulate muscle movement in mice

Researchers at Stanford University were able to use light to induce normal patterns of muscle contraction, in a study involving bioengineered mice whose nerve-cell surfaces are coated with special light-sensitive proteins.

The new approach allows scientists to more accurately reproduce muscle firing order, making it a valuable research tool. The investigators, from Stanford's Schools of Medicine and of Engineering, also believe this technique could someday spawn practical applications, from restoring movement to limbs paralysed by stroke or spinal-cord or brain injury to countering spasticity caused by cerebral palsy.

The study, to be published online Sept. 26 in Nature Medicine, employed a technology known as optogenetics, which involves the insertion of a specialised gene derived from algae into the genomes of experimental animals. This gene encodes a light-sensitive protein that situates itself on nerve-cell surfaces. Particular wavelengths of light can trigger nerve activity in animals endowed with these proteins, modifying nerve cells' firing patterns at the experimenters' will.

'Our group's focus is on restoring optimal movement for people with physical disabilities,' said one of the study's two senior authors, Scott Delp, PhD, a professor of bioengineering and the Clark Professor in the School of Engineering. 'With optical stimulation, we were able to reproduce the natural firing order of motor-nerve fibres - an important step forward.'

Optogenetics was invented at Stanford by the study's other senior author, Karl Deisseroth, MD, PhD, associate professor of bioengineering and of psychiatry and behavioural science, who has used optogenetics in many experiments to conduct research on the central nervous system of freely moving animals. 'This marks the first time the technique has been applied to the mammalian peripheral nervous system,' Deisseroth said.

The peripheral nervous system includes the long nerve fibres that exit the spinal cord to innervate skeletal muscle, producing voluntary movement. Skeletal muscles work as aggregations of what physiologists call 'motor units,' each consisting of a single nerve fibre plus the muscle fibres it innervates. At various points along the motor nerve, individual fibres exit the nerve to make contact with a variable number of skeletal-muscle fibres.

Motor units come in a variety of sizes. Small ones have single, thin nerve fibres that innervate several muscle fibres, whereas the lone, thicker nerve fibre in a larger motor unit may innervate several thousand of them. Normally, when motion is initiated, it takes stronger stimulation to 'fire' thick nerve fibres than thin ones. Thus, the smaller, so-called 'slow-twitch' muscle fibres start contracting before larger 'fast-twitch' fibres.

Fast-twitch fibres are essential for powerful athletic motions such as running, but fatigue quickly as they burn through finite stores of their primary fuel, glycogen. Their more diminutive slow-twitch counterparts, which burn their fuel slowly, are crucial to delicate movements such as those involved in sewing or drawing, as well as for fine-tuning coarser, more powerful movements. Activities relying mainly on small slow-twitch fibres can proceed for long periods of time, while larger but more-fatigable fast-twitch fibres are reserved for brief bursts of high-powered activity.

Previous attempts to restore lost motor function using programmed sequences of electrical impulses, delivered via a cuff placed around a nerve, have enabled paralysed people to walk, if only for a few minutes. Unfortunately, large nerve fibres are more responsive than smaller ones to electrical stimulation, so muscles contract in the wrong order - large, fast-twitch fibres first, then small, slow-twitch ones; this results in jerky motion and, soon thereafter, fatigue.

For the Nature Medicine study, lead author Michael Llewellyn, PhD, of Delp's lab, fashioned an 'optical cuff' lined with tiny, inward-facing light-emitting diodes, which could be placed around the bioengineered animals' sciatic nerves. The LEDs emitted blue light at intensities high enough to penetrate deep into the nerve, ensuring that all of its constituent nerve fibres would receive adequate stimulation from brief impulses of light from the LEDs. The investigators then showed that optical stimulation reproduced the proper firing order of muscle fibres, inducing contractions similar to those that take place under normal conditions.

Next, using various measures, the researchers compared optically induced muscle contractions with those induced by the electrical cuff. Small, slow-twitch muscle fibres were activated at the lowest levels of optical stimulation. But with electrical stimulation, bigger fibres were triggered first. What's more, optically triggered contractions were sustained far longer than those produced by electrical stimulation.

'With optical stimulation, the muscles retained about one-third of their initial maximum force after 20 minutes, and remained at that plateau for quite a while afterward,' said Llewellyn, who is now finishing his work on an MD at Stanford. 'Electrical stimulation completely exhausted the same muscles within four minutes.' Consistent with this, optical stimulation initiated contractions much more easily in muscles composed of predominantly slow-twitch fibres than in muscles richer in fast-twitch fibres. Electrical stimulation, in contrast, induced contractions equally in both muscle types.

The approach is, for now, primarily a research tool, Delp said. But it holds promise for clinical applications in the longer term if a way can be found to safely introduce genes coding for light-sensitive nerve-cell-surface proteins into people, he said. Just as techniques now use electrical cuffs to get paraplegics to walk for a few minutes, optical cuffs could be inserted microsurgically at appropriate places along motor nerve bundles, so that computer algorithm-controlled light impulses could induce firing in different fibres at different times, mimicking natural physiology.

Delp and Deisseroth are conducting similar research with a different protein that, in response to light, inhibits nerve fibres instead of triggering impulses in them, in the hope of someday being able to control spasticity, as for example occurs in cerebral palsy.